Prime Editing — Parameter Table
Cross-hypothesis reusable parameter table for prime editing and related precision editing strategies. Primary consumer: h07 (Prime Editing for STRC E1659A).
Editing efficiency in post-mitotic cells (in vivo / ex vivo)
Paper Value Units Conditions PMID Anzalone et al. 2019 7.1 % (sorted GFP+ nuclei) PE2, primary mouse cortical neurons, DNMT1 locus, lentiviral delivery 31634902 Zhi et al. 2022 Not quantified in abstract — Dual-AAV8 split-PE1024, adult mouse retina, Dnmt1, subretinal injection 34298129 Davis/Banskota et al. 2024 42 % (unenriched) Dual-AAV8 v3em PE-AAV, adult mouse cortex, in vivo 37142705 Davis/Banskota et al. 2024 46 % (unenriched) Dual-AAV8 v3em PE-AAV, adult mouse liver, in vivo 37142705 Davis/Banskota et al. 2024 11 % (unenriched) Dual-AAV8 v3em PE-AAV, adult mouse heart, in vivo 37142705 Roman et al. 2025 ~35 % (average) PE4, human iPSC-derived cardiomyocytes, RBM20-P633L, ex vivo 41210585
Adenine base editing in cochlear OHCs
Paper Value Units Conditions PMID Wang et al. 2025 48.5 % in vitro SchABE8e, cell culture, Pou4f3-Q113* 40968144 Wang et al. 2025 Near-complete hearing recovery Neonatal mouse, Anc80L65-SchABE8e, DFNA15 model, 4+ months 40968144
Adenine transversion editing (ACBE — A→C; WRONG direction for Misha’s STRC variant)
Paper Value Units Conditions PMID Chen et al. 2024 (evolved mAAG) 73 % max Cell culture, A→C transversion, mAAG-TadA8e-nCas9 evolved 37322276 Chen et al. 2024 (embryos) 44–56 % average Mouse embryos, founder alleles 37322276 ACBE payload size ~130 kDa Vs PE3 ~180 kDa; enables single-AAV 37322276
STRC gene replacement (dual-AAV; parallel track to h07 PE)
Paper Value Units Conditions PMID Shubina-Oleinik et al. 2021 Restored STRC expression — Dual-AAV9-PHP.B, Strc-KO neonatal mice, OHCs 34910522 Shubina-Oleinik et al. 2021 Normalized hair bundle — Morphology rescue in OHCs 34910522 Shubina-Oleinik et al. 2021 Substantially enhanced cochlear amplification — DPOAE improvement; exact % in PMC8673757 full text 34910522
Payload size constraints
System Size Units Single-AAV? Notes PE3 (PE2 + RT + pegRNA) ~6.3 kb No Dual-AAV split required AAV packaging limit ~4.7 kb — Standard rAAV ACBE ~130 kDa (protein) Yes mAAG-TadA8e-nCas9 fusion ABE (SchABE8e) <4.7 kb kb Yes (single) Fits single AAV
No published OHC prime editing data exists as of 2026-04. Plausible range for h07: 15–40% based on:
Lower bound: Anzalone 2019 neurons 7.1% (PE2, sorted, lentiviral — not fully representative)
Mid range: Roman 2025 cardiomyocytes ~35% (PE4, ex vivo)
Upper bound: Davis 2024 cortex 42% (dual-AAV v3em, in vivo)
This extrapolation is explicitly flagged in h07 as unverified. Key unknown: OHC-specific AAV transduction efficiency with the dual-AAV split-PE architecture.
Update protocol
Pull values from paper notes in papers/. Do not hardcode from memory. When OHC PE data becomes available, add row here + update h07 hub.
Connections