STRC pLDDT Profile and Cut Point Analysis
Source Data
CIF file: ~/DeepResearch/strc/af3-results/job-b-full-strc-homodimer/fold_full_strc_homodimer_model_2.cif
Chain A, 1775 residues. Per-residue pLDDT extracted from B-factor column.
Region Averages
| Region | Residues | Avg pLDDT | Classification |
|---|---|---|---|
| N-terminal | 1-114 | 40.9 | Very low confidence (disordered) |
| Middle | 115-615 | 50.2 | Low confidence (mostly disordered) |
| Transition zone | 616-699 | 60.2 | Medium confidence (partially ordered) |
| Shorter mini-STRC | 700-1775 | 71.2 | Confident (well-structured) |
| C-term only | 1075-1775 | 73.0 | Confident |
The Disorder Dip at Residues 685-694
Critical finding that justifies the 700 cut point:
| Residue | pLDDT | Note |
|---|---|---|
| 685 | 39.2 | Entering dip |
| 688 | 35.1 | Deep disorder |
| 691 | 31.2 | Minimum (lowest in transition zone) |
| 694 | 35.8 | Still low |
| 700 | 57.1 | Recovery point (our cut) |
| 706 | 73.2 | Confident folding begins |
| 714 | 78.0 | Fully structured |
The dip at 685-694 is a natural domain boundary: disorder valley between the transition zone and the LRR domain. Cutting at 700 captures the exact recovery point where confidence returns.
pLDDT Chart on Website
SVG chart with:
- 133 merged color rects (AF3 color scheme: red < 50, yellow 50-70, cyan 70-90, blue > 90)
- Smoothed line (window 10, 888 data points = every 2nd residue)
- Cut lines at 616, 700, 1075
- E1659 marker
- Legend
Generated from CIF data via Python script. Published on strc.egor.lol.
Data Files
- Raw pLDDT JSON:
/tmp/strc_plddt_profile.json - Smoothed data:
/tmp/strc_plddt_smoothed.json - Chart data:
/tmp/strc_chart_data.json - Final chart HTML:
/tmp/plddt_chart_final.html
Connections
[supports]STRC Mini-STRC Single-Vector Hypothesis — pLDDT data proves N-terminal is disordered- STRC AAV Vector Design — cut point at residue 700
[source]STRC AlphaFold3 Computational Experiments — job-b full homodimer[about]Misha