Matsuda et al. 2004 — N-linked glycosylation sites of prestin

Full citation: Matsuda K, Zheng J, Du GG, Klöcker N, Madison LD, Dallos P. “N-linked glycosylation sites of the motor protein prestin: effects on membrane targeting and electrophysiological function.” J Neurochem. 2004 May;89(4):928-38. PMID 15140192.

Numbers that matter

FindingValueNotes
N-glycosylation sitesN163 and N166Confirmed by site-directed mutagenesis N→Q
N166 vs N163 glycosylation extentN166 greater glycosylationDifferential programs for synthesis and trimming
Effect of N163/N166 removalAffects membrane targetingReduced prestin at plasma membrane
Electrophysiological impactReduced NLC (nonlinear capacitance)Prestin’s voltage-dependent motor function impaired

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